New COP1‐binding motifs involved in ER retrieval
WEBDec 1, 1998 · Here we report the identification of a new family of COP1‐binding motifs unrelated to dilysine or FF motifs, and we analyze its effect on the transport of tagged proteins in the early secretory pathway. These results illustrate the role of COP1‐mediated sorting in the secretory pathway.
Protease-controlled secretion and display of intercellular signals
WEBFeb 17, 2022 · Here we create a modular, generalizable design called R etained E ndoplasmic C lea vable Se cretion (RELEASE), with engineered proteins retained in the endoplasmic reticulum and...
Regulation of protein secretion through chemical regulation of ...
WEBMar 14, 2022 · Regulation of the ER escape represents a platform for the design of fast-responsive and tightly-controlled modular and scalable protein secretion system for mammalian cells.
Analysis of ER Resident Proteins in S. cerevisiae: Implementation …
WEBIn this study, we designed a series of PCR-based modules including codon-optimized epitopes and FP variants complete with C-terminal H/KDEL retrieval motifs. These conserved sequences are inherent to most soluble ER resident proteins.
Cholesterol-binding motifs in STING that control endoplasmic
WEBMar 29, 2024 · Collectively, we demonstrate that ER cholesterol sets a threshold for STING signalling through cholesterol-binding motifs in STING and we propose that this could be exploited for cancer...
Identification and characterization of an endoplasmic reticulum ...
WEBApr 22, 2016 · Sorting motifs are involved in the transport of diverse proteins. In the present study, we identified a hydrophobic peptide (WRPWRNFWWSIRVPWRRN) that was able to target enhanced green fluorescent protein- or DsRed2-enriched vesicular-like sub-compartments of the endoplasmic reticulum (ER).
A signal capture and proofreading mechanism for the KDEL ... - eLife
WEBJun 17, 2021 · Canonical ER retrieval signals can be broken down into two components: the −4 position, which enables the receptor to distinguish between different populations of ER proteins, and a tri-carboxylate moiety formed by the −3 aspartate, −2 glutamate and −1 C-terminal carboxylate.
Retention mechanisms for ER and Golgi membrane proteins
WEBAug 1, 2014 · In this opinion article, we first compare the possible functional differences of the well-known KKXX motif for COPI-mediated retrograde transport to the ER with the recently discovered KXD/E motif for possible retrograde transport …
Genetic variations within the ERE motif modulate plasticity and ...
WEBMar 15, 2011 · 21-mer DNA oligos containing the consensus ERE motif (AGGTCAnnnTGACCT) and all possible symmetric single nucleotide variants were commercially obtained from Sigma Genosys. The design of such oligos and their numbering relative to the central 3-bp spacer is illustrated in Fig. 1.
A di-arginine ER retention signal regulates trafficking of HCN1 ...
WEBAug 21, 2014 · The HCN1 di-arginine ER retention signal regulates the amount of channel trafficked to the cell surface. Di-arginine motifs found in a number of channels and receptors are thought to mediate constitutive ER retention …