Decoding the epitranscriptional landscape from native RNA
WEBJan 25, 2021 · The well-known DRACH/RRACH motif was localized and identified, consistent with previous studies, using differential analysis of ELIGOS to study the impact of RNA m6A methyltransferase by comparing wild type and knockouts in …
The m6A Consensus Motif Provides a Paradigm of …
WEBMay 10, 2021 · Abstract. To date more than 150 kinds of RNA chemical modifications have been identified in cellular RNAs, among which N6-methyladenosine (m6A) is the most prevalent mRNA modification in higher eukaryotes. m6A is widely conserved among eukaryotes and tends to occur in a RRACH consensus motif.
Structural insights into the RNA methyltransferase domain of
WEBMar 28, 2018 · Malone, T., Blumenthal, R. M. & Cheng, X. Structure-guided analysis reveals nine sequence motifs conserved among DNA amino-methyltransferases, and suggests a catalytic mechanism for these enzymes.
Accurate detection of m6A RNA modifications in native RNA …
WEBSep 9, 2019 · For this aim, we first focused our analysis on 5-mers that matched the known m 6 A motif RRACH, as these would be the most relevant in which to detect m 6 A modifications.
Dynamic landscape and evolution of m6A methylation in human
WEBMay 14, 2020 · To examine the motif dynamics and regulation between tissues, we divided the RRACH motif into four sub-motifs (GGACH, AGACH, GAACH and AAACH) for analysis. We found that different tissues had different sub-motif preferences (Figure 3D).
Decoding the epitranscriptional landscape from native RNA …
WEBJan 1, 2021 · The well-known DRACH/RRACH motif was localized and identified, consistent with previous studies, using differential analysis of ELIGOS to study the impact of RNA m 6 A methyltransferase by comparing wild …
Deep learning modeling m6A deposition reveals the importance of ...
WEBMay 17, 2022 · The m 6 A enhancers mostly include part of the RRACH motif and the m 6 A silencers generally contain CG/GT/CT motifs. Our finding is supported by both independent experimental validations and...
Single-base mapping of m 6 A by an antibody-independent …
WEBJul 3, 2019 · Here, we developed a precise and high-throughput antibody-independent m 6 A identification method based on the m 6 A-sensitive RNA endoribonuclease recognizing ACA motif (m 6 A-sensitive RNA-Endoribonuclease–Facilitated sequencing or m 6 …
Dynamic regulation and functions of mRNA m6A modification
WEBJan 29, 2022 · This review focuses on the effects of m6A modification on mRNA metabolism. In early works, RNA m6A was found on the consensus RNA motif RRACH (R = A or G; H = A, U, or C) with enrichment near stop …
The Biogenesis and Precise Control of RNA m6A Methylation
WEBSequence analysis revealed that m 6 A modification congruously occurs in the consensus motif RRACH (R= G or A; H= A, C or U; A could be converted to m 6 A) [5, 6]. Despite the prevalence of RRACH sequences in the transcriptome, only 1 …
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