Intrathecal Injections - Experimental Techniques - Pain Researcher
Dec 20, 2019 · Does any body have a protocol or advice for doing intrathecal injections in mice that doesn’t require surgery. Is there a way to inject directly into the lumbar enlargement (or other …
Intraganglionic injections into mouse DRG? - Protocols - Pain Researcher
Jul 11, 2016 · Have you ever heard or seen or done an intrathecal injection of an AAV construct with any neuron specific promoter which then goes to the DRG and then travels along peripheral nerves to the …
Death after intrathecal capsaicin? - Pain Researcher
Nov 16, 2023 · I inject only 7’5ul per animal, which is a quantity that is in the range reported by papers that use intrathecal capsaicin at that concentrations. I though that dose was safe but now knowing …
Intrathecal siRNA injection for spinal neuron gene knockdown - Your ...
Nov 3, 2016 · While I have never done intrathecal injections, I do a lot of nerve injections and we usually allow around 2 days for retrograde transport, after that we perform our injury and it works most of the …
Direct Intrathecal Injection of Recombinant Adeno-associated Viruses …
Mar 5, 2022 · Topic Replies Views Activity Intraspinal injection of adeno-associated viruses into the adult mouse spinal Experimental Techniques 0 355 September 4, 2021 Intrathecal Injections Experimental …
Intraspinal injection of adeno-associated viruses into the adult mouse ...
Sep 4, 2021 · Genetic dissection of neural circuits has been accelerated by recent advances in viral-based vectors. This protocol describes an effective approach to… Topic Replies Views Activity Direct …
Astrocytes transduction in the spinal cord - Experimental Techniques ...
Aug 9, 2018 · Have one tried lentivirus for astrocytes transduction in the spinal cord? Could intrathecal injection be used? @rurongji, @achamess
Intraganglionic injections into mouse DRG? - Page 2 - Protocols - Pain ...
May 8, 2024 · Dear Juan Carlos, Did you finally conducted the experiment involving the intrathecal injection of AAV9-hSyn-DIO-hM3Dq-mCherry in Nav1.8-Cre animals? I am currently attempting a …
Systemic Administration of AAV PHP.S for Delivery to DRG
Sep 23, 2021 · I am planning to use AAV-PHP.S and/or AAV-MaCPNS1/2 for transducing DRG neurons. Have you tried IP admin at P1? How was the transduction efficiency? There are papers …
EF1a promoter to drive expression in DRG in vivo?
Nov 17, 2017 · I finally tried AAV-EF1a intrathecal injections and the infection wasn’t successful in my cre animals. I tried AAV9-EF1α-mCherry-FLEX-DTA (homemade, not great titer) and AAV9-EF1α …